Chemical composition:
The leaves contain 0.6 to 1% volatile oil. The main component of the oil is α-thujone, including thujene, fechone, pinene, and caryphyChemicalbookllene. ), camphor, bornyl acetate, terpene alcohol, etc.; flavonoids include citrin, quercetin, myricin, cypress biflavonoids, cypress biflavonoids, etc. The crude total flavonoid content of fresh Platycladus orientalis leaves is 1.72%. It also contains tannins, resins, vitamin C and other ingredients.
Extraction method
1. Microwave extraction: Microwave has an obvious synergistic effect on extracting flavonoids from Platycladus orientalis leaves, reducing the amount of extractant, shortening the extraction time and improving the yield. It has the advantages of time saving, high efficiency and energy saving, thereby reducing the extraction cost. , which is superior to the traditional direct heating extraction method in many aspects. The most suitable process conditions are: φ (ethanol) 60%, material-liquid mass ratio 1:20,
microwave time 4min, microwave power 385W. A verification test was conducted under this process condition, and the yield of Platycladus orientalis leaf extract was as high as 5.905%. Microwave extraction can greatly shorten the extraction time, reduce the amount of extractant, and increase the extract yield.
2. Extraction liquid type: In the Chemicalbook process of extracting flavonoids from Platycladus orientalis leaves using methanol, ethanol or ethyl acetate as the extraction liquid, extraction time and extraction temperature are the main factors that affect the extraction effect. Using methanol as the extraction liquid, the optimal extraction plan is: extraction time 6 hours, extraction temperature 70oC, liquid-to-material ratio 8:1, and total flavonoid yield 8.450%. Using absolute ethanol as the extraction solution, the optimal extraction plan is: extraction time 6 hours, extraction temperature 85 degrees Celsius, liquid-to-material
ratio 10:1, and total flavonoid yield 7.422%. Using ethyl acetate as the extraction liquid, the optimal extraction plan is: extraction time 6 hours, extraction temperature 90oC, liquid-to-material ratio 6:1, and total flavonoid yield 6.748%. Comparing the three extraction solutions, the total flavonoids extracted using methanol as the solvent has the highest yield, and the methanol extraction temperature is the lowest and the energy consumption is the least. Therefore, the extraction process using methanol as the solvent is the best.